Coding

Part:BBa_K3900002:Design

Designed by: Jonas Dauser   Group: iGEM21_Bielefeld-CeBiTec   (2021-09-21)


Mutated Ribose Binding Protein (RBP) to bind BTCA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
    Illegal NgoMIV site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Increased affinity


Source

iGEM21_Bielefeld-CeBiTec

References